A universal rolling circle amplification for label-free and highly specific nucleic acid sensing.
Chem Commun (Camb)
; 58(84): 11863-11866, 2022 Oct 20.
Article
in En
| MEDLINE
| ID: mdl-36196746
ABSTRACT
Traditional RCA methods face some drawbacks including limited specificity and amplification templates with sequence dependence. Herein, a universal RCA (URCA) strategy for label-free nucleic acid sensing with high specificity was proposed, which could be used for sensing of different nucleic acids without redesigning or synthesizing new amplification templates. The URCA strategy also showed high accuracy for miRNA analysis in practical samples.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nucleic Acids
/
MicroRNAs
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2022
Document type:
Article
Affiliation country: