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A universal rolling circle amplification for label-free and highly specific nucleic acid sensing.
Sun, Mengxu; Liu, Birong; Li, Tong; Li, Chunrong; Duan, Wen-Jun; Xie, Baoping; Li, Min-Min; Chen, Jin-Xiang; Dai, Zong; Chen, Jun.
Affiliation
  • Sun M; NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China. chenj258@smu.edu.cn.
  • Liu B; NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China. chenj258@smu.edu.cn.
  • Li T; NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China. chenj258@smu.edu.cn.
  • Li C; Qiannan Medical College for Nationalities, Duyun, 558000, P. R. China.
  • Duan WJ; NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China. chenj258@smu.edu.cn.
  • Xie B; NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China. chenj258@smu.edu.cn.
  • Li MM; Center of Clinical Laboratory, The First Affiliated Hospital of Jinan University, Guangzhou 510632, P. R. China. limm269@126.com.
  • Chen JX; NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China. chenj258@smu.edu.cn.
  • Dai Z; Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen 518107, P. R. China.
  • Chen J; NMPA Key Laboratory for Research and Evaluation of Drug Metabolism, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China. chenj258@smu.edu.cn.
Chem Commun (Camb) ; 58(84): 11863-11866, 2022 Oct 20.
Article in En | MEDLINE | ID: mdl-36196746
ABSTRACT
Traditional RCA methods face some drawbacks including limited specificity and amplification templates with sequence dependence. Herein, a universal RCA (URCA) strategy for label-free nucleic acid sensing with high specificity was proposed, which could be used for sensing of different nucleic acids without redesigning or synthesizing new amplification templates. The URCA strategy also showed high accuracy for miRNA analysis in practical samples.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nucleic Acids / MicroRNAs Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2022 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nucleic Acids / MicroRNAs Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2022 Document type: Article Affiliation country: