Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol.
Biotechnol Lett
; 44(12): 1389-1400, 2022 Dec.
Article
in En
| MEDLINE
| ID: mdl-36203106
ABSTRACT
OBJECTIVES:
1,5-pentanediamine (cadaverine) is a C5 platform chemical, also an important raw material for bio-polyamide PA5X. With increasing concerns about the depletion of fossil resources and global environmental protection, cadaverine bio-production has attracted more attentions.RESULTS:
Here, a microbial consortium consisting of Corynebacterium glutamicum cgl-FDK and Escherichia coli BL-ABST-Spy was constructed to de novo synthesize cadaverine utilizing glycerol as the sole carbon resource. The glycerol utilization pathway was initially constructed in C. glutamicum cgl-FDK to produce lysine from glycerol. Then, the pyridoxal 5'-phosphate (PLP) biosynthesis pathway and SpyTag/SpyCatcher protein-ligation system for lysine decarboxylase (CadA) and cadaverine-lysine antiporter protein (CadB) were introduced into E. coli BL-ABST-Spy to synthesize cadaverine from lysine. Furthermore, the fermentation conditions of microbial consortium were optimized and the cadaverine production reached 9.3 g/L with glycerol as the sole carbon source.CONCLUSIONS:
This work provides a promising strategy for efficiently producing cadaverine from glycerol with an artificial microbial consortium.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Corynebacterium glutamicum
/
Glycerol
Language:
En
Journal:
Biotechnol Lett
Year:
2022
Document type:
Article