Nucleic acid aptamers as aptasensors for plant biology.
Trends Plant Sci
; 28(3): 359-371, 2023 03.
Article
in En
| MEDLINE
| ID: mdl-36357246
ABSTRACT
Our knowledge of cell- and tissue-specific quantification of phytohormones is heavily reliant on laborious mass spectrometry techniques. Genetically encoded biosensors have allowed spatial and some temporal quantification of phytohormones intracellularly, but there is still limited information on their intercellular distributions. Here, we review nucleic acid aptamers as an emerging biosensing platform for the detection and quantification of analytes with high affinity and specificity. Options for DNA aptamer technology are explained through selection, sequencing analysis and techniques for evaluating affinity and specificity, and we focus on previously developed DNA aptamers against various plant analytes. We suggest how these tools might be applied in planta for quantification of molecules of interest both intracellularly and intercellularly.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nucleic Acids
/
Biosensing Techniques
/
Aptamers, Nucleotide
Language:
En
Journal:
Trends Plant Sci
Journal subject:
BOTANICA
Year:
2023
Document type:
Article