An optical system for noninvasive microscopy of psoriatic mice in vivo.
J Biophotonics
; 16(4): e202200310, 2023 04.
Article
in En
| MEDLINE
| ID: mdl-36519190
ABSTRACT
Psoriasis is a chronic inflammatory skin disease involved with both complex morphological changes of skin and immune processes. The clinical diagnostics and research of psoriasis often require invasive biopsy which lacks their real-time dynamics in vivo. Here we report a noninvasive microscopic system developed by combining in vivo fluorescent microscopy, optical clearing, and immunolabeling to enable real-time imaging of immune cells and cytokines in blood flow in psoriatic animal models. The vascular morphology and time-lapse kinetics of interleukin (IL)-23, IL-17, tumor necrosis factor-α, and CD4+ cells in blood are captured at submicron resolution through the thickening epidermis and opaque scales during the development of psoriasis in vivo. Our data suggest IL-23 recruits CD4+ cells to release IL-17 in blood that further leaks out in the psoriatic skin area. This optical system enables noninvasive and real-time assessment of immune molecules and cells in vivo, providing good potential for medical researches on psoriasis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Psoriasis
/
Skin
/
Optical Imaging
/
Microscopy, Fluorescence
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Biophotonics
Journal subject:
BIOFISICA
Year:
2023
Document type:
Article
Affiliation country: