ALDEFLUOR activity, ALDH isoforms, and their clinical significance in cancers.
J Enzyme Inhib Med Chem
; 38(1): 2166035, 2023 Dec.
Article
in En
| MEDLINE
| ID: mdl-36651035
ABSTRACT
High aldehyde dehydrogenase (ALDH) activity is a metabolic feature of adult stem cells and various cancer stem cells (CSCs). The ALDEFLUOR system is currently the most commonly used method for evaluating ALDH enzyme activity in viable cells. This system is applied extensively in the isolation of normal stem cells and CSCs from heterogeneous cell populations. For many years, ALDH1A1 has been considered the most important subtype among the 19 ALDH family members in determining ALDEFLUOR activity. However, in recent years, studies of many types of normal and tumour tissues have demonstrated that other ALDH subtypes can also significantly influence ALDEFLUOR activity. In this article, we briefly review the relationships between various members of the ALDH family and ALDEFLUOR activity. The clinical significance of these ALDH isoforms in different cancers and possible directions for future studies are also summarised.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aldehyde Dehydrogenase
/
Neoplasms
Limits:
Adult
/
Humans
Language:
En
Journal:
J Enzyme Inhib Med Chem
Journal subject:
BIOQUIMICA
/
QUIMICA
Year:
2023
Document type:
Article
Affiliation country: