Direct observation of motor protein stepping in living cells using MINFLUX.
Science
; 379(6636): 1010-1015, 2023 03 10.
Article
in En
| MEDLINE
| ID: mdl-36893247
ABSTRACT
Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dimensions using the recently introduced super-resolution technique MINFLUX. Using this approach, we resolved the precise stepping motion of the motor protein kinesin-1 as it walked on microtubules in living cells. Nanoscopic tracking of motors walking on the microtubules of fixed cells also enabled us to resolve the architecture of the microtubule cytoskeleton with protofilament resolution.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cells
/
Kinesins
/
Microscopy, Fluorescence
/
Microtubules
Limits:
Humans
Language:
En
Journal:
Science
Year:
2023
Document type:
Article
Affiliation country: