Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine.
STAR Protoc
; 4(2): 102310, 2023 May 13.
Article
in En
| MEDLINE
| ID: mdl-37182207
ABSTRACT
The small intestine is an excellent model for studying changes in vasculature in response to different diseases or gene deletions. Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for perfusion fixation, tissue sample preparation, immunofluorescence staining, and whole-mount preparation of stained samples. Our protocol will enable researchers to visualize and analyze the intricate network of vessels in the small intestine. For complete details on the use and execution of this protocol, please refer to Karaman et al. (2022).1.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
STAR Protoc
Year:
2023
Document type:
Article
Affiliation country: