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Genotypic and Phenotypic Characterization of Escherichia coli Isolates Recovered from the Uterus of Mares with Fertility Problems.
Nocera, Francesca Paola; Maurizi, Linda; Masullo, Angelo; Nicoletti, Mauro; Conte, Antonietta Lucia; Brunetti, Francesca; De Martino, Luisa; Zagaglia, Carlo; Longhi, Catia.
Affiliation
  • Nocera FP; Department of Veterinary Medicine and Animal Production, University of Naples 'Federico II', Via F. Delpino 1, 80137 Naples, Italy.
  • Maurizi L; Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
  • Masullo A; Department of Veterinary Medicine and Animal Production, University of Naples 'Federico II', Via F. Delpino 1, 80137 Naples, Italy.
  • Nicoletti M; Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
  • Conte AL; Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
  • Brunetti F; Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
  • De Martino L; Department of Veterinary Medicine and Animal Production, University of Naples 'Federico II', Via F. Delpino 1, 80137 Naples, Italy.
  • Zagaglia C; Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
  • Longhi C; Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00185 Rome, Italy.
Animals (Basel) ; 13(10)2023 May 14.
Article in En | MEDLINE | ID: mdl-37238068
ABSTRACT
Escherichia coli is the bacterial pathogen most frequently associated with mare infertility. Here, we characterized 24 E. coli strains isolated from mares which presented signs of endometritis and infertility from a genotypic and phenotypic point of view. The majority of the isolates belonged to phylogenetic group B1 (9/24, 37.5%). Regarding antibiotic resistance profiles, 10 out of 24 (41.7%) were multidrug-resistant (MDR). Moreover, 17 out of 24 (70.8%) were strong or moderate biofilm producers, and of these eight were MDR strains. Interestingly, 21 out of 24 (87.5%) E. coli strains were phenotypically resistant to ampicillin and 10 of them were also resistant to amoxicillin with clavulanic acid. Regarding the presence of selected virulence factors, 50% of the examined strains carried at least three of them, with fimH detected in all strains, and followed by kpsMTII (11/24, 45.9%). No strain was able to invade HeLa cell monolayers. No relevant differences for all the investigated characteristics were shown by strains that grew directly on plates versus strains requiring the broth-enrichment step before growing on solid media. In conclusion, this work provides new insight into E. coli strains associated with mares' infertility. These results broaden the knowledge of E. coli and, consequently, add useful information to improve prevention strategies and therapeutic treatments contributing to a significant increase in the pregnancy rate in mares.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Animals (Basel) Year: 2023 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Animals (Basel) Year: 2023 Document type: Article Affiliation country:
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