A functional group-guided approach to aptamers for small molecules.
Science
; 380(6648): 942-948, 2023 06 02.
Article
in En
| MEDLINE
| ID: mdl-37262137
ABSTRACT
Aptameric receptors are important biosensor components, yet our ability to identify them depends on the target structures. We analyzed the contributions of individual functional groups on small molecules to binding within 27 target-aptamer pairs, identifying potential hindrances to receptor isolation-for example, negative cooperativity between sterically hindered functional groups. To increase the probability of aptamer isolation for important targets, such as leucine and voriconazole, for which multiple previous selection attempts failed, we designed tailored strategies focused on overcoming individual structural barriers to successful selections. This approach enables us to move beyond standardized protocols into functional group-guided searches, relying on sequences common to receptors for targets and their analogs to serve as anchors in regions of vast oligonucleotide spaces wherein useful reagents are likely to be found.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biosensing Techniques
/
Aptamers, Nucleotide
/
SELEX Aptamer Technique
/
Voriconazole
/
Leucine
/
Antifungal Agents
Language:
En
Journal:
Science
Year:
2023
Document type:
Article
Affiliation country: