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Generation and characterisation of a human induced pluripotent stem cell line, NIMHi007-A, from peripheral blood mononuclear cells derived from an adult healthy female.
Nimonkar, Madhura Milind; Arunachal, Gautham; Dash, Suravi Sasmita; Kandy, Swapna Kannothum; Raghavendra, Kenchaiah; Chetan, Ghati K; Mehta, Bhupesh; Markandeya, Yogananda S.
Affiliation
  • Nimonkar MM; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India.
  • Arunachal G; Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India.
  • Dash SS; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India.
  • Kandy SK; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India.
  • Raghavendra K; Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India.
  • Chetan GK; Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India.
  • Mehta B; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India.
  • Markandeya YS; Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India. Electronic address: yogananda@nimhans.ac.in.
Stem Cell Res ; 70: 103130, 2023 08.
Article in En | MEDLINE | ID: mdl-37269666
ABSTRACT
We report the generation and characterisation of a human induced pluripotent stem cell (iPSC) line, NIMHi007-A, derived from peripheral blood mononuclear cells (PBMCs) of a healthy female adult individual. PBMCs were reprogrammed using the non-integrating Sendai virus consisting of Yamanaka reprogramming factors- SOX2, cMYC, KLF4, and OCT4. The iPSCs displayed a normal karyotype, express pluripotency markers, and could generate into three germ layers, endoderm, mesoderm, and ectoderm, in-vitro. This iPSC line, NIMHi007-A, can be used as a healthy control for various in-vitro disease models and study their underlying pathophysiological mechanisms.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Induced Pluripotent Stem Cells Type of study: Prognostic_studies Limits: Adult / Female / Humans Language: En Journal: Stem Cell Res Year: 2023 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Induced Pluripotent Stem Cells Type of study: Prognostic_studies Limits: Adult / Female / Humans Language: En Journal: Stem Cell Res Year: 2023 Document type: Article Affiliation country: