Efficient clathrin-mediated entry of enteric adenoviruses in human duodenal cells.
J Virol
; 97(10): e0077023, 2023 10 31.
Article
in En
| MEDLINE
| ID: mdl-37823645
ABSTRACT
IMPORTANCE Enteric adenoviruses have historically been difficult to grow in cell culture, which has resulted in lack of knowledge of host factors and pathways required for infection of these medically relevant viruses. Previous studies in non-intestinal cell lines showed slow infection kinetics and generated comparatively low virus yields compared to other adenovirus types. We suggest duodenum-derived HuTu80 cells as a superior cell line for studies to complement efforts using complex intestinal tissue models. We show that viral host cell factors required for virus entry differ between cell lines from distinct origins and demonstrate the importance of clathrin-mediated endocytosis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenoviridae
/
Clathrin
/
Endocytosis
/
Virus Internalization
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Virol
Year:
2023
Document type:
Article
Affiliation country: