Experimental and Computational Approaches to Measure Telomere Length: Recent Advances and Future Directions.
Curr Hematol Malig Rep
; 18(6): 284-291, 2023 Dec.
Article
in En
| MEDLINE
| ID: mdl-37947937
ABSTRACT
PURPOSE OF REVIEW The length of telomeres, protective structures at the chromosome ends, is a well-established biomarker for pathological conditions including multisystemic syndromes called telomere biology disorders. Approaches to measure telomere length (TL) differ on whether they estimate average, distribution, or chromosome-specific TL, and each presents their own advantages and limitations. RECENT FINDINGS:
The development of long-read sequencing and publication of the telomere-to-telomere human genome reference has allowed for scalable and high-resolution TL estimation in pre-existing sequencing datasets but is still impractical as a dedicated TL test. As sequencing costs continue to fall and strategies for selectively enriching telomere regions prior to sequencing improve, these approaches may become a promising alternative to classic methods. Measurement methods rely on probe hybridization, qPCR or more recently, computational methods using sequencing data. Refinements of existing techniques and new approaches have been recently developed but a test that is accurate, simple, and scalable is still lacking.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Telomere
Limits:
Humans
Language:
En
Journal:
Curr Hematol Malig Rep
Journal subject:
HEMATOLOGIA
/
NEOPLASIAS
Year:
2023
Document type:
Article
Affiliation country: