Visualizing the DNA repair process by a photolyase at atomic resolution.
Science
; 382(6674): eadd7795, 2023 12.
Article
in En
| MEDLINE
| ID: mdl-38033054
ABSTRACT
Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX). We obtained 18 snapshots that show time-dependent changes in four reaction loci. We used these results to create a movie that depicts the repair of CPD lesions in the picosecond-to-nanosecond range, followed by the recovery of the enzymatic moieties involved in catalysis, completing the formation of the fully reduced enzyme-product complex at 500 nanoseconds. Finally, back-flip intermediates of the thymine bases to reanneal the DNA were captured at 25 to 200 microseconds. Our data cover the complete molecular mechanism of a photolyase and, importantly, its chemistry and enzymatic catalysis at work across a wide timescale and at atomic resolution.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pyrimidine Dimers
/
Methanosarcina
/
Archaeal Proteins
/
Deoxyribodipyrimidine Photo-Lyase
/
DNA Repair
Language:
En
Journal:
Science
Year:
2023
Document type:
Article
Affiliation country: