Transcriptional regulation of FACT involves Coordination of chromatin accessibility and CTCF binding.
J Biol Chem
; 300(1): 105538, 2024 Jan.
Article
in En
| MEDLINE
| ID: mdl-38072046
ABSTRACT
Histone chaperone FACT (facilitates chromatin transcription) is well known to promote chromatin recovery during transcription. However, the mechanism how FACT regulates genome-wide chromatin accessibility and transcription factor binding has not been fully elucidated. Through loss-of-function studies, we show here that FACT component Ssrp1 is required for DNA replication and DNA damage repair and is also essential for progression of cell phase transition and cell proliferation in mouse embryonic fibroblast cells. On the molecular level, absence of the Ssrp1 leads to increased chromatin accessibility, enhanced CTCF binding, and a remarkable change in dynamic range of gene expression. Our study thus unequivocally uncovers a unique mechanism by which FACT complex regulates transcription by coordinating genome-wide chromatin accessibility and CTCF binding.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatin
/
High Mobility Group Proteins
/
Gene Expression Regulation
/
DNA-Binding Proteins
/
Histone Chaperones
/
CCCTC-Binding Factor
Limits:
Animals
Language:
En
Journal:
J Biol Chem
Year:
2024
Document type:
Article