Hydroalcoholic extract of Scrophularia striata has a significant therapeutic effect on thioacetamide-induced liver cirrhosis in rats.
Horm Mol Biol Clin Investig
; 44(4): 371-377, 2023 Dec 01.
Article
in En
| MEDLINE
| ID: mdl-38124628
ABSTRACT
OBJECTIVES:
Liver cirrhosis is one of the most important causes of death from liver diseases. Nowadays, the use of herbal medicines has increased due to its availability, less side effects and cheapness for the treatment of liver diseases. The present study was conducted to examine therapeutic effects of hydroalcoholic extract of Scrophularia striata (S. striata) on thioacetamide-induced liver cirrhosis in rats through evaluate its effects on oxidative stress markers and the expression of metalloproteinase 1 (TIMP 1), toll-like receptor-4 (TLR-4), and Mitofusin (MFN2) genes.METHODS:
24 male rats were selected by simple random sampling. Rats were randomly assigned to four groups group I healthy rats, group II thioacetamide (TAA) injected rats, group III TAA injected rats+100â¯mg/kgâ¯bw of S. striata and group IV TAA injected rats+200â¯mg/kgâ¯bw of S. striata. Liver cirrhosis was induced in rats by a 300â¯mg/kgâ¯bw TAA administration twice with an interval of 24â¯h. After 8 weeks of treatment by S. striata at doses of 100 and 200â¯mg/kgâ¯bw, biochemical factors and oxidative stress markers (SOD, TAC, GPX, CAT and MDA) were measured using spectrophotometric methods. Also, gene expression of TIMP 1, TLR-4, and MFN2 were analyzed using real-time PCR. ANOVA and Bonferroni post hoc test analysis were applied to evaluate the data.RESULTS:
The results showed the S. striata extract significantly improve the serum ALT, AST and ALP levels, TIMP 1, TLR-4, and MFN2 genes and oxidative stress markers (SOD, TAC, GPX, CAT and MDA) in the liver tissues when compared to control group (p<0.05). Also, it was found that the beneficial effects of the S. striata were dose-dependent.CONCLUSIONS:
Based on the results obtained S. striata by reducing the expression of TIMP 1, TLR-4, and MFN2 genes and improving oxidative stress might be used as adjuvant treatment for liver cirrhosis.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Scrophularia
/
Liver Diseases
Limits:
Animals
Language:
En
Journal:
Horm Mol Biol Clin Investig
Year:
2023
Document type:
Article
Affiliation country: