Furosemide attenuates tubulointerstitial injury and allows functional testing of porcine kidneys during normothermic machine perfusion.
Artif Organs
; 48(6): 595-605, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38164041
ABSTRACT
BACKGROUND:
Normothermic machine perfusion (NMP) is a promising pretransplant kidney quality assessment platform, but it remains crucial to increase its diagnostic potential while ensuring minimal additional injury to the already damaged kidney. Interventions that alter tubular transport can influence renal function and injury during perfusion. This study aimed to determine whether furosemide and desmopressin affect renal function and injury during NMP.METHODS:
Eighteen porcine kidneys (n = 6 per group) were subjected to 30 min of warm ischemia and 4 h of oxygenated hypothermic perfusion before being subjected to 6 h of NMP. Each organ was randomized to receive no drug, furosemide (750 mg), or desmopressin (16 µg) during NMP.RESULTS:
Compared with the other groups, the addition of furosemide resulted in significantly increased urine output, fractional excretion of sodium and potassium, and urea clearance during NMP. Urinary neutrophil gelatinase-associated lipocalin levels decreased significantly with furosemide supplementation compared with the other groups. The addition of desmopressin did not result in any significantly different outcome measurements compared with the control group.CONCLUSIONS:
This study showed that the addition of furosemide affected renal function while attenuating tubulointerstitial injury during NMP. Therefore, furosemide supplementation may provide renal protection and serve as a functional test for pretransplant kidney viability assessment during NMP.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Organ Preservation
/
Perfusion
/
Furosemide
/
Kidney
Type of study:
Clinical_trials
Limits:
Animals
Language:
En
Journal:
Artif Organs
/
Artif. organs
/
Artificial organs
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: