A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations.
Nat Commun
; 15(1): 729, 2024 Jan 25.
Article
in En
| MEDLINE
| ID: mdl-38272895
ABSTRACT
Aedes aegypti is the main vector of several major pathogens including dengue, Zika and chikungunya viruses. Classical mosquito control strategies utilizing insecticides are threatened by rising resistance. This has stimulated interest in new genetic systems such as gene drivesHere, we test the regulatory sequences from the Ae. aegypti benign gonial cell neoplasm (bgcn) homolog to express Cas9 and a separate multiplexing sgRNA-expressing cassette inserted into the Ae. aegypti kynurenine 3-monooxygenase (kmo) gene. When combined, these two elements provide highly effective germline cutting at the kmo locus and act as a gene drive. Our target genetic element drives through a cage trial population such that carrier frequency of the element increases from 50% to up to 89% of the population despite significant fitness costs to kmo insertions. Deep sequencing suggests that the multiplexing design could mitigate resistance allele formation in our gene drive system.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aedes
/
Zika Virus
/
Zika Virus Infection
/
Gene Drive Technology
/
Insecticides
Limits:
Animals
Language:
En
Journal:
Nat Commun
/
Nature communications
Journal subject:
BIOLOGIA
/
CIENCIA
Year:
2024
Document type:
Article
Country of publication: