Antioxidant, AChE inhibitory, and anticancer effects of Verbascum thapsus extract.
Cell Mol Biol (Noisy-le-grand)
; 69(14): 211-216, 2023 Dec 20.
Article
in En
| MEDLINE
| ID: mdl-38279434
ABSTRACT
Verbascum thapsus (VT) is a medicinal plant that is used in folk medicine to treat a variety of ailments. For this study, the biological functions of VT methanol extract were determined in vitro. The plant's methanol extract was created through the maceration process. The phytochemical composition of plant extracts was investigated using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The antioxidant capacity of the extract was determined using the DPPH (2,2-diphenyl-1-picrylhydrazil) and ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) tests and its cytotoxicity was assessed using the MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole)) assay on the Caco-2 (human colorectal adenocarcinoma cells), LNCaP (Lymph Node Carcinoma of the Prostate), and HEK293 cell lines (Human embryonic kidney 293 cells) used to model colon, prostate, and non-cancerous cells. VT extract showed low DPPH and ABTS radical scavenging activities compared to standard antioxidants at 30 mg/ml concentration. In addition, it was determined that VT extract inhibited acetylcholinesterase enzyme.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sulfonic Acids
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Verbascum
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Benzothiazoles
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Antioxidants
Limits:
Humans
/
Male
Language:
En
Journal:
Cell Mol Biol (Noisy-le-grand)
Journal subject:
BIOLOGIA MOLECULAR
Year:
2023
Document type:
Article
Affiliation country:
Country of publication: