Quantitative assessment of transforming growth factor-ß1 release from dentin matrix upon conditioning with ethylene diamine tetra-acetate, doxycycline hydrochloride, and propolis: An in vitro study.
J Conserv Dent Endod
; 26(5): 564-568, 2023.
Article
in En
| MEDLINE
| ID: mdl-38292370
ABSTRACT
Background:
Transforming growth factor-beta 1 (TGF-ß1) is a key morphogen in regenerative endodontics that plays a central role in regulating cellular functions. Various chelating agents have been shown to release this growth factor upon conditioning. The objective of the study was to evaluate TGF-ß1 release from the dentin matrix upon conditioning with ethylene diamine tetra-acetate (EDTA), doxycycline hydrochloride, and propolis. Materials andMethods:
Forty-two human 3rd molar teeth were collected and coronal portion of the teeth was sectioned to obtain dentin blocks with dimensions 2 mm × 2 mm × 2 mm. The blocks were then randomly divided into three groups depending on the conditioning agent used; Group 1 17% EDTA, Group 2 doxycycline hydrochloride (100 mg/mL), and Group 3 propolis (250 µg/mL). Conditioned blocks were placed in 0.5 mL of phosphate buffered saline and incubated for 1 week for quantification.Results:
Highest TGF-ß1 release was noted for propolis (0.21 ng/mL), followed by doxycycline hydrochloride (0.18 ng/mL) and 17% EDTA (0.14 ng/mL).Conclusion:
Doxycycline hydrochloride and propolis significantly enhanced the release of TGF-ß1 from the dentin matrix compared to EDTA (P < 0.05). No significant difference was observed between doxycycline hydrochloride and propolis (P > 0.05). Doxycycline and propolis can be used as effective alternatives to EDTA during regenerative endodontic procedures.
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Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Conserv Dent Endod
Year:
2023
Document type:
Article
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