Modified trichrome stain for faster and improved detection of intestinal protozoan parasites.
Trop Doct
; 54(2): 139-146, 2024 Apr.
Article
in En
| MEDLINE
| ID: mdl-38311979
ABSTRACT
Permanent stains such as trichrome have better sensitivity but are time-consuming and the fixative includes toxic mercuric chloride. Thus, a newer modification was tested and found to be a superior, faster and safer staining technique for intestinal parasitic detection. Our study lasted 9 months and a single stool sample was collected from each enrolled patient. We evaluated classical trichrome (T1 - using Schaudinn fixative) with newer modifications, which involved different fixatives with mordant combinations (T2 - acetic acid + hydrated aluminium sulphate, T3 - citric acid + copper sulphate hydrate). Conventional PCR targeting Entamoeba histolytica, Giardia lamblia and Cryptosporidium spp. was taken as the reference. Out of 175 stool samples, 25.1% protozoa were identified by wet mount, 24% by each T1 and T2, 25.7% by T3. Statistically, T3 and T2 had higher sensitivity as compared to T1 and wet mount when PCR was used as reference.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Parasites
/
Azo Compounds
/
Eosine Yellowish-(YS)
/
Cryptosporidiosis
/
Cryptosporidium
/
Entamoeba histolytica
/
Intestinal Diseases, Parasitic
/
Methyl Green
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Trop Doct
Year:
2024
Document type:
Article
Affiliation country: