Mechanistic insights into the C-type lectin receptor CLEC12A-mediated immune recognition of monosodium urate crystal.
J Biol Chem
; 300(3): 105765, 2024 Mar.
Article
in En
| MEDLINE
| ID: mdl-38367667
ABSTRACT
CLEC12A, a member of the C-type lectin receptor family involved in immune homeostasis, recognizes MSU crystals released from dying cells. However, the molecular mechanism underlying the CLEC12A-mediated recognition of MSU crystals remains unclear. Herein, we reported the crystal structure of the human CLEC12A-C-type lectin-like domain (CTLD) and identified a unique "basic patch" site on CLEC12A-CTLD that is necessary for the binding of MSU crystals. Meanwhile, we determined the interaction strength between CLEC12A-CTLD and MSU crystals using single-molecule force spectroscopy. Furthermore, we found that CLEC12A clusters at the cell membrane and seems to serve as an internalizing receptor of MSU crystals. Altogether, these findings provide mechanistic insights for understanding the molecular mechanisms underlying the interplay between CLEC12A and MSU crystals.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Uric Acid
/
Receptors, Mitogen
/
Lectins, C-Type
Limits:
Humans
Language:
En
Journal:
J Biol Chem
Year:
2024
Document type:
Article
Country of publication: