Investigating Heterogeneous Cell-Cycle Progression Using Single-Cell Imaging Approaches.
Methods Mol Biol
; 2740: 263-273, 2024.
Article
in En
| MEDLINE
| ID: mdl-38393481
ABSTRACT
Investigating cell-cycle progression has been challenging due to the complex interconnectivity of regulatory processes and inherent cell-to-cell heterogeneity, which often require synchronization procedures. However, recent advancements in cell-cycle sensors and single-cell imaging techniques have turned this heterogeneity into an advantage for investigating the molecular mechanisms underlying diverse responses. This has led to significant progress in our understanding of cell-cycle regulation. In this paper, we present a comprehensive live single-cell imaging workflow that leverages cutting-edge live-cell sensors. These advanced single-cell imaging procedures provide promising opportunities for elucidating the molecular mechanisms underpinnings of heterogeneous responses in cell-cycle progression.
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Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Division
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
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