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Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds.
Oliver-Urrutia, Carolina; Diez-Escudero, Anna; Sumbalova Koledova, Zuzana; Celko, Ladislav; Hailer, Nils P; Montufar, Edgar B.
Affiliation
  • Oliver-Urrutia C; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
  • Diez-Escudero A; Department of Surgical Sciences - Orthopaedics, Uppsala University Hospital, Uppsala, Sweden.
  • Sumbalova Koledova Z; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
  • Celko L; Laboratory of Tissue Morphogenesis and Cancer, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
  • Hailer NP; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
  • Montufar EB; Department of Surgical Sciences - Orthopaedics, Uppsala University Hospital, Uppsala, Sweden.
Methods Mol Biol ; 2764: 43-60, 2024.
Article in En | MEDLINE | ID: mdl-38393588
ABSTRACT
In vitro cell cultures are a very useful tool for the validation of biomaterial cytocompatibility, especially for bone tissue engineering scaffolds and bone implants. In this chapter, a protocol for a static three-dimensional osteoblast cell culture on titanium scaffolds and subsequent analysis of osteogenic capacity is presented. The protocol is explained for additively manufactured titanium scaffolds, but it can be extrapolated to other scaffolds with similar size and structure, while differing in composition or manufactured technology. Additionally, the protocol can be used for culture of other adherent cell types beyond osteoblast cells such as mesenchymal stem cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Titanium / Printing, Three-Dimensional Language: En Journal: Methods Mol Biol / Methods in molecular biology / Methods mol. biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Country of publication:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Titanium / Printing, Three-Dimensional Language: En Journal: Methods Mol Biol / Methods in molecular biology / Methods mol. biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Country of publication: