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Using Liver Organoids as Models to Study the Pathobiology of Rare Liver Diseases.
Obeid, Dalia A; Mir, Tanveer Ahmad; Alzhrani, Alaa; Altuhami, Abdullah; Shamma, Talal; Ahmed, Sana; Kazmi, Shadab; Fujitsuka, Iriya; Ikhlaq, Mohd; Shabab, Mohammad; Assiri, Abdullah M; Broering, Dieter C.
Affiliation
  • Obeid DA; Tissue/Organ Bioengineering and BioMEMS Lab, Organ Transplant Centre of Excellence, Transplant Research and Innovation Department, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia.
  • Mir TA; Tissue/Organ Bioengineering and BioMEMS Lab, Organ Transplant Centre of Excellence, Transplant Research and Innovation Department, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia.
  • Alzhrani A; Tissue/Organ Bioengineering and BioMEMS Lab, Organ Transplant Centre of Excellence, Transplant Research and Innovation Department, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia.
  • Altuhami A; College of Medicine, Alfaisal University, Riyadh 11533, Saudi Arabia.
  • Shamma T; College of Applied Medical Sciences, King Abdulaziz University, Jeddah 21423, Saudi Arabia.
  • Ahmed S; Tissue/Organ Bioengineering and BioMEMS Lab, Organ Transplant Centre of Excellence, Transplant Research and Innovation Department, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia.
  • Kazmi S; Tissue/Organ Bioengineering and BioMEMS Lab, Organ Transplant Centre of Excellence, Transplant Research and Innovation Department, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia.
  • Fujitsuka I; Tissue/Organ Bioengineering and BioMEMS Lab, Organ Transplant Centre of Excellence, Transplant Research and Innovation Department, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia.
  • Ikhlaq M; School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi 923-1292, Ishikawa, Japan.
  • Shabab M; School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi 923-1292, Ishikawa, Japan.
  • Assiri AM; Department of Child Health, School of Medicine, University of Missouri, Columbia, MO 65212, USA.
  • Broering DC; Toyama Red Cross Hospital, Toyama 930-0859, Toyama, Japan.
Biomedicines ; 12(2)2024 Feb 17.
Article in En | MEDLINE | ID: mdl-38398048
ABSTRACT
Liver organoids take advantage of several important features of pluripotent stem cells that self-assemble in a three-dimensional culture matrix and reproduce many aspects of the complex organization found within their native tissue or organ counterparts. Compared to other 2D or 3D in vitro models, organoids are widely believed to be genetically stable or docile structures that can be programmed to virtually recapitulate certain biological, physiological, or pathophysiological features of original tissues or organs in vitro. Therefore, organoids can be exploited as effective substitutes or miniaturized models for the study of the developmental mechanisms of rare liver diseases, drug discovery, the accurate evaluation of personalized drug responses, and regenerative medicine applications. However, the bioengineering of organoids currently faces many groundbreaking challenges, including a need for a reasonable tissue size, structured organization, vascularization, functional maturity, and reproducibility. In this review, we outlined basic methodologies and supplements to establish organoids and summarized recent technological advances for experimental liver biology. Finally, we discussed the therapeutic applications and current limitations.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Biomedicines Year: 2024 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Biomedicines Year: 2024 Document type: Article Affiliation country: