High-Throughput Screening for the Prevalence of Neutralizing Antibodies against Human Adenovirus Serotype 5.
Vaccines (Basel)
; 12(2)2024 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-38400138
ABSTRACT
Adenoviral vectors based on the human adenovirus species C serotype 5 (HAdV-C5) are commonly used for vector-based gene therapies and vaccines. In the preclinical stages of development, their safety and efficacy are often validated in suitable animal models. However, pre-existing neutralizing antibodies may severely influence study outcomes. Here, we generated a new HAdV-C5-based reporter vector and established a high-throughput screening assay for the multivalent detection of HAdV-C5-neutralizing antibodies in serum. We screened the sera of rhesus macaques at different primate centers, and of rabbits, horses, cats, and dogs, showing that HAdV-C5-neutralizing antibodies can be found in all species, albeit at different frequencies. Our results emphasize the need to prescreen model animals in HAdV-C5-based studies.
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Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Vaccines (Basel)
Year:
2024
Document type:
Article
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