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Dual "on-off" signal conversion strategy based on surface plasmon coupling and resonance energy transfer for visual electrochemiluminescence ratiometric analysis of MiRNA-141.
Wang, Yin-Zhu; Wang, Ling-Ling; Liu, Yan; Zhang, Yu-Qi; Li, Meng-Li; Chen, Chuan-Xiang; Zhu, Jia-Wan; Yang, Fu; Hu, Yong-Hong.
Affiliation
  • Wang YZ; College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211800, PR China. Electronic address: wangyz@just.edu.cn.
  • Wang LL; College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211800, PR China; College of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212100, PR China.
  • Liu Y; College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211800, PR China.
  • Zhang YQ; College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211800, PR China.
  • Li ML; College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211800, PR China.
  • Chen CX; College of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212100, PR China. Electronic address: cxchen@just.edu.cn.
  • Zhu JW; College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211800, PR China; College of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212100, PR China.
  • Yang F; College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211800, PR China; College of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212100, PR China.
  • Hu YH; College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211800, PR China. Electronic address: yonghonghuyg@163.com.
Biosens Bioelectron ; 253: 116162, 2024 Jun 01.
Article in En | MEDLINE | ID: mdl-38437748
ABSTRACT
An electrochemiluminescence (ECL) biosensor with a pair of new ECL emitters and a novel sensing mechanism was designed for the high-sensitivity detection of microRNA-141 (miRNA-141). Sulfur-doped boron nitrogen quantum dots (S-BN QDs) were initially employed to modify the cathode of the bipolar electrode (BPE), while the anode reservoir was [Ir(dfppy)2(bpy)]PF6/TPrA system. The next step involved attaching H1-bound ultra-small WO3-x nanodots (WO3-x NDs) to the S-BN QDs-modified BPE cathode via DNA hybridization. A strong surface plasmon coupling (SPC) effect was observed between S-BN QDs and WO3-x NDs, which allowed for the enhancement of the red and visible ECL emission from S-BN QDs. After target-induced cyclic amplification to produce abundant Zn2+ and Au NPs-DNA3-Au NPs (Au NPs-S3-Au NPs), Zn2+ could cleave DNA at a nucleotide sequence-specific recognition site to release the WO3-x NDs, resulting in the first diminution of cathode ECL signal and the first enhancement of anode ECL signal. Moreover, the ECL signal at cathode decreased for the second time and the emission of [Ir(dfppy)2(bpy)]PF6 was continuously enhanced after the introduction of Au nanoparticles-S3-Au nanoparticles on the cathode surface. Our sensing mode with a dual "on-off" signal conversion strategy shows a good detection capability for miRNAs ranging from 10-17 to 10-10 M, with a limit of detection (LOD) as low as 10-17 M, which has great application potential in biomedical research and clinical diagnosis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biosensing Techniques / MicroRNAs / Metal Nanoparticles Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biosensing Techniques / MicroRNAs / Metal Nanoparticles Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article