Protocol for studying topological DNA interactions by purified fission yeast condensin.
STAR Protoc
; 5(2): 102995, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38578833
ABSTRACT
To understand the transition from interphase chromatin into well-shaped chromosomes during cell divisions, we need to understand the biochemical activities of the contributing proteins. Here, we present a protocol to investigate how the ring-shaped condensin complex sequentially and topologically entraps two DNA substrates. We describe the steps to prepare purified Schizosaccharomyces pombe condensin, as well as bulk biochemical assays to monitor the first and second DNA capture reactions. This protocol may facilitate further investigations of these essential genome organizers. For complete details on the use and execution of this protocol, please refer to Tang et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Schizosaccharomyces
/
Adenosine Triphosphatases
/
Multiprotein Complexes
/
DNA-Binding Proteins
Language:
En
Journal:
STAR Protoc
/
STAR protocols
Year:
2024
Document type:
Article
Country of publication: