3D printed ß-tricalcium phosphate versus synthetic bone mineral scaffolds: A comparative in vitro study of biocompatibility.
Biomed Mater Eng
; 35(4): 365-375, 2024.
Article
in En
| MEDLINE
| ID: mdl-38578877
ABSTRACT
BACKGROUND:
ß-tricalcium phosphate (ß-TCP) has been successfully utilized as a 3D printed ceramic scaffold in the repair of non-healing bone defects; however, it requires the addition of growth factors to augment its regenerative capacity. Synthetic bone mineral (SBM) is a novel and extrudable carbonate hydroxyapatite with ionic substitutions known to facilitate bone healing. However, its efficacy as a 3D printed scaffold for hard tissue defect repair has not been explored.OBJECTIVE:
To evaluate the biocompatibility and cell viability of human osteoprecursor (hOP) cells seeded on 3D printed SBM scaffolds via in vitro analysis.METHODS:
SBM and ß-TCP scaffolds were fabricated via 3D printing and sintered at various temperatures. Scaffolds were then subject to qualitative cytotoxicity testing and cell proliferation experiments utilizing (hOP) cells.RESULTS:
SBM scaffolds sintered at lower temperatures (600 °C and 700 °C) induced greater levels of acute cellular stress. At higher sintering temperatures (1100 °C), SBM scaffolds showed inferior cellular viability relative to ß-TCP scaffolds sintered to the same temperature (1100 °C). However, qualitative analysis suggested that ß-TCP presented no evidence of morphological change, while SBM 1100 °C showed few instances of acute cellular stress.CONCLUSION:
Results demonstrate SBM may be a promising alternative to ß-TCP for potential applications in bone tissue engineering.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Materials Testing
/
Calcium Phosphates
/
Cell Survival
/
Cell Proliferation
/
Tissue Scaffolds
/
Printing, Three-Dimensional
Limits:
Humans
Language:
En
Journal:
Bio-medical materials and engineering
/
Biomed Mater Eng
/
Biomed. mater. eng
Journal subject:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: