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Kinesin KIF3A regulates meiotic progression and spindle assembly in oocyte meiosis.
Liu, Jing-Cai; Pan, Zhen-Nan; Ju, Jia-Qian; Zou, Yuan-Jing; Pan, Meng-Hao; Wang, Yue; Wu, Xin; Sun, Shao-Chen.
Affiliation
  • Liu JC; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
  • Pan ZN; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
  • Ju JQ; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
  • Zou YJ; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
  • Pan MH; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
  • Wang Y; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
  • Wu X; State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
  • Sun SC; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. sunsc@njau.edu.cn.
Cell Mol Life Sci ; 81(1): 168, 2024 Apr 08.
Article in En | MEDLINE | ID: mdl-38587639
ABSTRACT
Kinesin family member 3A (KIF3A) is a microtubule-oriented motor protein that belongs to the kinesin-2 family for regulating intracellular transport and microtubule movement. In this study, we characterized the critical roles of KIF3A during mouse oocyte meiosis. We found that KIF3A associated with microtubules during meiosis and depletion of KIF3A resulted in oocyte maturation defects. LC-MS data indicated that KIF3A associated with cell cycle regulation, cytoskeleton, mitochondrial function and intracellular transport-related molecules. Depletion of KIF3A activated the spindle assembly checkpoint, leading to metaphase I arrest of the first meiosis. In addition, KIF3A depletion caused aberrant spindle pole organization based on its association with KIFC1 to regulate expression and polar localization of NuMA and γ-tubulin; and KIF3A knockdown also reduced microtubule stability due to the altered microtubule deacetylation by histone deacetylase 6 (HDAC6). Exogenous Kif3a mRNA supplementation rescued the maturation defects caused by KIF3A depletion. Moreover, KIF3A was also essential for the distribution and function of mitochondria, Golgi apparatus and endoplasmic reticulum in oocytes. Conditional knockout of epithelial splicing regulatory protein 1 (ESRP1) disrupted the expression and localization of KIF3A in oocytes. Overall, our results suggest that KIF3A regulates cell cycle progression, spindle assembly and organelle distribution during mouse oocyte meiosis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oocytes / Kinesins Limits: Animals Language: En Journal: Cell Mol Life Sci Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oocytes / Kinesins Limits: Animals Language: En Journal: Cell Mol Life Sci Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Country of publication: