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RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells.
Xia, Xian; Sung, Po-Yu; Martynowycz, Michael W; Gonen, Tamir; Roy, Polly; Zhou, Z Hong.
Affiliation
  • Xia X; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA; California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Sung PY; Department of Infection Biology, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK.
  • Martynowycz MW; Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Gonen T; Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, CA 90095, USA.
  • Roy P; Department of Infection Biology, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK.
  • Zhou ZH; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA; California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: hong.zhou@ucla.edu.
Cell ; 187(9): 2236-2249.e17, 2024 Apr 25.
Article in En | MEDLINE | ID: mdl-38614100
ABSTRACT
Unlike those of double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and ssRNA viruses, the mechanism of genome packaging of dsRNA viruses is poorly understood. Here, we combined the techniques of high-resolution cryoelectron microscopy (cryo-EM), cellular cryoelectron tomography (cryo-ET), and structure-guided mutagenesis to investigate genome packaging and capsid assembly of bluetongue virus (BTV), a member of the Reoviridae family of dsRNA viruses. A total of eleven assembly states of BTV capsid were captured, with resolutions up to 2.8 Å, with most visualized in the host cytoplasm. ATPase VP6 was found underneath the vertices of capsid shell protein VP3 as an RNA-harboring pentamer, facilitating RNA packaging. RNA packaging expands the VP3 shell, which then engages middle- and outer-layer proteins to generate infectious virions. These revealed "duality" characteristics of the BTV assembly mechanism reconcile previous contradictory co-assembly and core-filling models and provide insights into the mysterious RNA packaging and capsid assembly of Reoviridae members and beyond.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Viral / Capsid / Bluetongue virus / Cryoelectron Microscopy / Capsid Proteins / Viral Genome Packaging Limits: Animals Language: En Journal: Cell Year: 2024 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Viral / Capsid / Bluetongue virus / Cryoelectron Microscopy / Capsid Proteins / Viral Genome Packaging Limits: Animals Language: En Journal: Cell Year: 2024 Document type: Article Affiliation country: