[Age-dependent expression of HSP90 in the hippocampus of APP/PS1 mice].
Sheng Li Xue Bao
; 76(2): 257-265, 2024 Apr 25.
Article
in Zh
| MEDLINE
| ID: mdl-38658375
ABSTRACT
The present study aims to observe the change in expression of heat shock protein 90 (HSP90) along with amyloid-ß (Aß) and phosphorylated Tau (p-Tau) protein levels in the hippocampus tissue of Alzheimer's disease (AD) transgenic animal model with age. APP/PS1 transgenic mice at age of 6-, 9- and 12-month and C57BL/6J mice of the same age were used. The cognitive abilities of these animals were evaluated using a Morris water maze. Western blot or immunohistochemistry was used to detect the expressions of HSP90 and Aß1-42, as well as the phosphorylation levels of Tau protein in the hippocampus. The hsp90 mRNA levels and the morphology and number of cells in the hippocampus were detected with real-time quantitative polymerase chain reaction (qRT-PCR) and Nissl staining, respectively. The results showed that compared with C57BL/6J mice of the same age, HSP90 and hsp90 mRNA expression were decreased (P < 0.05 or P < 0.01), while Aß1-42 and p-Tau protein levels were increased (P < 0.05 or P < 0.01) in the hippocampal tissue of APP/PS1 transgenic mice. Meanwhile, the decrease in HSP90 and hsp90 mRNA expression (P < 0.05 or P < 0.01), the increase in Aß1-42 and p-Tau levels (P < 0.01 or P < 0.05) in hippocampal tissue and the reduction in behavioral ability showed a progressive development with the advancing of age in the APP/PS1 transgenic mice. In conclusion, in the hippocampal tissue of APP/PS1 mice, the decrease in HSP90 expression and the increase in Aß1-42 and p-Tau levels together with the decline of their cognitive ability are age-dependent.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mice, Transgenic
/
Amyloid beta-Peptides
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Amyloid beta-Protein Precursor
/
Tau Proteins
/
HSP90 Heat-Shock Proteins
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Alzheimer Disease
/
Hippocampus
/
Mice, Inbred C57BL
Limits:
Animals
Language:
Zh
Journal:
Sheng Li Xue Bao
Year:
2024
Document type:
Article
Affiliation country: