Massive field-of-view sub-cellular traction force videography enabled by Single-Pixel Optical Tracers (SPOT).
Biosens Bioelectron
; 258: 116318, 2024 Aug 15.
Article
in En
| MEDLINE
| ID: mdl-38701538
ABSTRACT
We report a massive field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13 mm2 at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells' mechanical traction force into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells. We used this platform to explore the mechanical wave propagation in a tightly connected sheet of Neonatal Rat Ventricular Myocytes (NRVMs) and discovered that the activation time of some tissue regions are heterogeneous from the overall spiral wave behavior of the cardiac wave.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Myocytes, Cardiac
Limits:
Animals
Language:
En
Journal:
Biosens Bioelectron
/
Biosens. bioelectron
/
Biosensors and bioelectronics
Journal subject:
BIOTECNOLOGIA
Year:
2024
Document type:
Article
Country of publication: