Salivary metabolomic identification of biomarker candidates for oral melanoma and oral squamous cell carcinoma in dogs.
J Vet Intern Med
; 38(4): 2293-2304, 2024.
Article
in En
| MEDLINE
| ID: mdl-38703129
ABSTRACT
BACKGROUND:
Oral melanoma (OM) and oral squamous cell carcinoma (OSCC) are frequently diagnosed in dogs, presenting a challenge in distinguishing them from benign oral tumors (BN). Salivary metabolomic biomarkers offer a practical solution because of saliva's direct contact with tumors and the noninvasive nature of collection.OBJECTIVE:
Assess the diversity and abundance of the salivary metabolome in dogs with BN, OM, and OSCC using amine/phenol submetabolome analysis and high-performance chemical isotope labeling liquid chromatography-mass spectrometry (CIL LC-MS). ANIMALS Study included 11 BN, 24 OM, 10 OSCC, and 20 healthy control dogs.METHODS:
Case-control cross-sectional study was conducted to assess salivary submetabolic profiles in dogs with BN, OM, and OSCC and healthy dogs. Samples were labeled with 12C-dansyl chloride and analyzed using CIL LC-MS targeted to amine- and phenol-containing metabolites for amine/phenol submetabolome analysis.RESULTS:
Distinct clusters and significant differences in metabolite concentrations were observed among the oral cancer, BN, and control groups. A total of 154 and 66 metabolites showed significantly altered concentrations, particularly in OM and OSCC, respectively, when compared with BN (Padj < .05). Potential metabolic biomarkers were identified for each cancer, including decreased concentrations of seryl-arginine and sarcosine in OSCC. Moreover, high-confidence putative metabolites were identified, including an increase in tryptophyl-threonine and a decrease in 1,2-dihydroxynapthalene-6-sulfonic acid and hydroxyprolyl-hydroxyproline for OM. CONCLUSIONS AND CLINICAL IMPORTANCE We identified high coverage of the amine/phenol submetabolome, including seryl-arginine, and sarcosine, in OSCC. Our findings emphasize the potential of these biomarkers for distinguishing between oral OSCC and BN in dogs.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saliva
/
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Biomarkers, Tumor
/
Dog Diseases
/
Melanoma
Limits:
Animals
Language:
En
Journal:
J Vet Intern Med
Journal subject:
MEDICINA INTERNA
/
MEDICINA VETERINARIA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: