Spectral flow cytometry: Fundamentals and future impact.
Methods Cell Biol
; 186: 311-332, 2024.
Article
in En
| MEDLINE
| ID: mdl-38705605
ABSTRACT
Spectral flow cytometry has emerged as a significant player in the cytometry marketplace, with the potential for rapid growth. Despite a slow start, the technology has made significant strides in advancing various areas of single-cell analysis utilized by the scientific community. The integration of spectral cytometry into clinical laboratories and diagnostic processes is currently underway and is expected to garner a significant level of widespread acceptance in the near future. However, incorporating a new methodological approach into existing research programs can lead to misunderstandings or even misuse. This chapter offers an introductory yet comprehensive explanation of the scientific principles that form the foundation of spectral cytometry. Specifically, it delves into the unmixing processes that are utilized for data analysis. This overview is designed for those who are new to the field and seeking an informative guide to this exciting emerging technology.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Single-Cell Analysis
/
Flow Cytometry
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Cell Biol
/
Methods cell biol
/
Methods in cell biology
Year:
2024
Document type:
Article
Country of publication: