Gastrodin ameliorates neuroinflammation in Alzheimer's disease mice by inhibiting NF-κB signaling activation via PPARγ stimulation.
Aging (Albany NY)
; 16(10): 8657-8666, 2024 05 15.
Article
in En
| MEDLINE
| ID: mdl-38752930
ABSTRACT
AIM:
We investigated the effects and targets of gastrodin (GAS) for improving cognitive ability in Alzheimer's disease (AD).METHODS:
The targets and mechanisms of GAS were analyzed by network pharmacology. Morris water and eight-arm radial mazes were used to detect the behaviors of 7-months-old APP/PS1 mice. The levels of IBA-1 and PPARγ were examined by histochemical staining, nerve cells were detected by Nissl staining, inflammatory cytokines were measured by ELISA, and protein expressions were monitored by Western blotting. The neurobehavioral effects of GAS on mice were detected after siRNA silencing of PPARγ. Microglia were cultured in vitro and Aß1-42 was used to simulate the pathology of AD. After treatment with GAS, the levels of inflammatory cytokines and proteins were assayed.RESULTS:
Network pharmacological analysis revealed that PPARγ was the action target of GAS. By stimulating PPARγ, GAS inhibited NF-κB signaling activation and decreased neuroinflammation and microglial activation, thereby ameliorating the cognitive ability of AD mice. After silencing PPARγ, GAS could not further improve such cognitive ability. Cellular-level results demonstrated that GAS inhibited microglial injury, reduced tissue inflammation, and activated PPARγ.CONCLUSIONS:
GAS can regulate microglia-mediated inflammatory response by stimulating PPARγ and inhibiting NF-κB activation, representing a mechanism whereby it improves the cognitive behavior of AD.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Benzyl Alcohols
/
Signal Transduction
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NF-kappa B
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Microglia
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PPAR gamma
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Alzheimer Disease
/
Glucosides
Limits:
Animals
Language:
En
Journal:
Aging (Albany NY)
Journal subject:
GERIATRIA
Year:
2024
Document type:
Article
Affiliation country: