Photodynamic treatment increases the lifespan and oxidative stress resistance of Caenorhabditis elegans.
Free Radic Biol Med
; 221: 98-110, 2024 Aug 20.
Article
in En
| MEDLINE
| ID: mdl-38754743
ABSTRACT
Photodynamic therapy is a noninvasive treatment in which specific photosensitizers and light are used to produce high amounts of reactive oxygen species (ROS), which can be employed for targeted tissue destruction in cancer treatment or antimicrobial therapy. However, it remains unknown whether lower amounts of ROS produced by mild photodynamic therapy increase lifespan and stress resistance at the organism level. Here, we introduce a novel photodynamic treatment (PDTr) that uses 20 µM hypericin, a photosensitizer that originates from Hypericum perforatum, and orange light (590 nm, 5.4 W/m2, 1 min) to induce intracellular ROS formation (ROS), thereby resulting in lifespan extension and improved stress resistance in C. elegans. The PDTr-induced increase in longevity was abrogated by N-acetyl cysteine, suggesting the hormetic response was driven by prooxidative mechanisms. PDTr activated the translocation of SKN-1/NRF-2 and DAF-16/FOXO, leading to elevated expression of downstream oxidative stress-responsive genes, including ctl-1, gst-4, and sod-3. In summary, our findings suggest a novel PDTr method that extends the lifespan of C. elegans under both normal and oxidative stress conditions through the activation of SKN-1 and DAF-16 via the involvement of many antioxidant genes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Perylene
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Photochemotherapy
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Transcription Factors
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Reactive Oxygen Species
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Photosensitizing Agents
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Caenorhabditis elegans
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Oxidative Stress
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Caenorhabditis elegans Proteins
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Longevity
Limits:
Animals
Language:
En
Journal:
Free Radic Biol Med
Journal subject:
BIOQUIMICA
/
MEDICINA
Year:
2024
Document type:
Article
Country of publication: