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Urinary Proteomic Biomarkers of Trabecular Bone Volume Change during Army Basic Combat Training.
Flanagan, Shawn D; Hougland, Juliana R; Zeng, Xuemei; Cantrell, Pamela S; Sun, Mai; Jones-Laughner, Jackie; Canino, Maria C; Hughes, Julie M; Foulis, Stephen A; Taylor, Kathryn M; Walker, Leila A; Guerriere, Katelyn I; Sterczala, Adam J; Connaboy, Chris; Beckner, Meaghan E; Matheny, Ronald W; Nindl, Bradley C.
Affiliation
  • Zeng X; Biomedical Mass Spectrometry Center, University of Pittsburgh, Pittsburgh, PA.
  • Cantrell PS; Biomedical Mass Spectrometry Center, University of Pittsburgh, Pittsburgh, PA.
  • Sun M; Biomedical Mass Spectrometry Center, University of Pittsburgh, Pittsburgh, PA.
  • Jones-Laughner J; Biomedical Mass Spectrometry Center, University of Pittsburgh, Pittsburgh, PA.
  • Canino MC; Department of Sports Medicine and Nutrition, School of Health and Rehabilitation Sciences, University of Pittsburgh, Pittsburgh, PA.
  • Hughes JM; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA.
  • Foulis SA; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA.
  • Taylor KM; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA.
  • Walker LA; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA.
  • Guerriere KI; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA.
  • Sterczala AJ; Department of Sports Medicine and Nutrition, School of Health and Rehabilitation Sciences, University of Pittsburgh, Pittsburgh, PA.
  • Beckner ME; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA.
  • Matheny RW; Military Operational Medicine Research Program, Fort Detrick, MD.
  • Nindl BC; Department of Sports Medicine and Nutrition, School of Health and Rehabilitation Sciences, University of Pittsburgh, Pittsburgh, PA.
Med Sci Sports Exerc ; 56(9): 1644-1654, 2024 Sep 01.
Article in En | MEDLINE | ID: mdl-38758530
ABSTRACT

PURPOSE:

The purpose of this study is to optimize a dMS-based urinary proteomic technique and evaluate the relationship between urinary proteome content and adaptive changes in bone microarchitecture during BCT.

METHODS:

Urinary proteomes were analyzed with an optimized dMS technique in two groups of 13 recruits ( N = 26) at the beginning (Pre) and end (Post) of BCT. Matched by age (21 ± 4 yr), sex (16 W), and baseline tibial trabecular bone volume fractions (Tb.BV/TV), these groups were distinguished by the most substantial (High) and minimal (Low) improvements in Tb.BV/TV. Differential protein expression was analyzed with mixed permutation ANOVA and false discovery proportion-based adjustment for multiple comparisons.

RESULTS:

Tibial Tb.BV/TV increased from pre- to post-BCT in High (3.30 ± 1.64%, P < 0.0001) but not Low (-0.35 ± 1.25%, P = 0.4707). The optimized dMS technique identified 10,431 peptides from 1368 protein groups that represented 165 integrative biological processes. Seventy-four urinary proteins changed from pre- to post-BCT ( P = 0.0019), and neutrophil-mediated immunity was the most prominent ontology. Two proteins (immunoglobulin heavy constant gamma 4 and C-type lectin domain family 4 member G) differed from pre- to post-BCT in High and Low ( P = 0.0006).

CONCLUSIONS:

The dMS technique can identify more than 1000 urinary proteins. At least 74 proteins are responsive to BCT, and other principally immune system-related proteins show differential expression patterns that coincide with adaptive bone formation.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biomarkers / Proteomics / Cancellous Bone / Military Personnel Limits: Adolescent / Adult / Female / Humans / Male Language: En Journal: Med Sci Sports Exerc / Med. sci. sports exerc / Medicine and science in sports and exercise Year: 2024 Document type: Article Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biomarkers / Proteomics / Cancellous Bone / Military Personnel Limits: Adolescent / Adult / Female / Humans / Male Language: En Journal: Med Sci Sports Exerc / Med. sci. sports exerc / Medicine and science in sports and exercise Year: 2024 Document type: Article Country of publication: