Urinary Proteomic Biomarkers of Trabecular Bone Volume Change during Army Basic Combat Training.
Med Sci Sports Exerc
; 56(9): 1644-1654, 2024 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-38758530
ABSTRACT
PURPOSE:
The purpose of this study is to optimize a dMS-based urinary proteomic technique and evaluate the relationship between urinary proteome content and adaptive changes in bone microarchitecture during BCT.METHODS:
Urinary proteomes were analyzed with an optimized dMS technique in two groups of 13 recruits ( N = 26) at the beginning (Pre) and end (Post) of BCT. Matched by age (21 ± 4 yr), sex (16 W), and baseline tibial trabecular bone volume fractions (Tb.BV/TV), these groups were distinguished by the most substantial (High) and minimal (Low) improvements in Tb.BV/TV. Differential protein expression was analyzed with mixed permutation ANOVA and false discovery proportion-based adjustment for multiple comparisons.RESULTS:
Tibial Tb.BV/TV increased from pre- to post-BCT in High (3.30 ± 1.64%, P < 0.0001) but not Low (-0.35 ± 1.25%, P = 0.4707). The optimized dMS technique identified 10,431 peptides from 1368 protein groups that represented 165 integrative biological processes. Seventy-four urinary proteins changed from pre- to post-BCT ( P = 0.0019), and neutrophil-mediated immunity was the most prominent ontology. Two proteins (immunoglobulin heavy constant gamma 4 and C-type lectin domain family 4 member G) differed from pre- to post-BCT in High and Low ( P = 0.0006).CONCLUSIONS:
The dMS technique can identify more than 1000 urinary proteins. At least 74 proteins are responsive to BCT, and other principally immune system-related proteins show differential expression patterns that coincide with adaptive bone formation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biomarkers
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Proteomics
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Cancellous Bone
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Military Personnel
Limits:
Adolescent
/
Adult
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Female
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Humans
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Male
Language:
En
Journal:
Med Sci Sports Exerc
/
Med. sci. sports exerc
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Medicine and science in sports and exercise
Year:
2024
Document type:
Article
Country of publication: