Protocol for changing gene expression in 3T3-L1 (pre)adipocytes using siRNA-mediated knockdown.
STAR Protoc
; 5(2): 103075, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38805394
ABSTRACT
3T3-L1 is a model cell line which can be differentiated from preadipocytes into mature adipocytes. Here, we present a protocol for changing gene expression in 3T3-L1 (pre)adipocytes using small interfering RNA (siRNA)-mediated knockdown. We describe steps to perform the knockdown of a certain gene prior to differentiation (day 4) to analyze the impact on adipogenesis. We then detail procedures for knockdown on day 8 of differentiation to study the role of a certain gene in mature adipocyte function. For complete details on the use and execution of this protocol, please refer to Kaczmarek et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
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Adipocytes
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RNA, Small Interfering
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3T3-L1 Cells
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Adipogenesis
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Gene Knockdown Techniques
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Affiliation country: