T-B coculture assay for functional analysis of antigen-specific memory CD4+ T cells.
STAR Protoc
; 5(2): 103119, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38850540
ABSTRACT
The B cell "help" function of CD4+ T cells is critical in establishing the humoral arm of adaptive immunity. Here, we present a protocol to measure the "help" function of antigen-specific memory T cells using an autologous T-B coculture supplemented with monocytes. We describe steps for cell preparation, human cell sorting, coculture, and a flow cytometry-based assessment of B cell outputs. This protocol demonstrates enhanced sensitivity and proves useful in evaluating T-B collaboration in various contexts of health and disease. For complete details on the use and execution of this protocol, please refer to Ansari et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
B-Lymphocytes
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CD4-Positive T-Lymphocytes
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Coculture Techniques
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Flow Cytometry
Limits:
Humans
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: