Aptamer-based electrochemical nanobiosensor for research and monitoring of multiple sclerosis in mice models.
Bioelectrochemistry
; 160: 108744, 2024 May 19.
Article
in En
| MEDLINE
| ID: mdl-38852383
ABSTRACT
Multiple sclerosis (MS) is a severe progressive autoimmune-inflammatory, demyelinating process in the central nervous system (CNS) with heterogeneous neurological symptoms appearing as a consequence of myelin break down. Myelin basic protein (MBP) makes up to 30 % of the CNS myelin [1] and it is known to be released into the cerebrospinal fluid (CSF) as a bioindicator of MS. Autoimmune encephalomyelitis (EAE) is a mice model of MS widely used for research and development of new treatments [2]. Herein, MBP specific aptamer developed for possible therapeutic purposes in mouse model [3] was applied as a bioreceptor for MBP recognition. A nanobiosensor for MBP detection and monitoring was developed by using graphene oxide (GO) nanoparticles integrated onto the screen-printed carbon electrodes (SPCE) and aptamer immobilized to create a bioactive layer on the sensor surface for MBP binding. The measurements were carried out using electrochemical impedance spectrometry (EIS). Validation studies were carried out in a biological matrix (artificial CSF) containing MBP, and MSA. The aptasensor had LOD in artificial CSF 0.01 ng/mL and showed its usability in the concentration range of 0.01
64 ng/mL.
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Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Bioelectrochemistry
Journal subject:
BIOQUIMICA
Year:
2024
Document type:
Article
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