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Integrated morphometric and molecular approaches to screen hybrid from wild Labeo rohita and Labeo catla parent populations.
Lenka, Pritam; Singh, Namrata; Ghosh, Deepra; Mahadani, Pradosh; Ghosh, Sajalendu.
Affiliation
  • Lenka P; Department of Zoology, Dr. Shyama Prasad Mukherjee University, Ranchi, Jharkhand, India.
  • Singh N; Department of Zoology, Dr. Shyama Prasad Mukherjee University, Ranchi, Jharkhand, India.
  • Ghosh D; Department of Statistics, Fox School of Business, Temple University, Philadelphia, PA, 19122, USA.
  • Mahadani P; College of Biotechnology, Birsa Agricultural University, Ranchi, Jharkhand, India.
  • Ghosh S; Department of Zoology, Dr. Shyama Prasad Mukherjee University, Ranchi, Jharkhand, India. ghosh.sajal@gmail.com.
Mol Biol Rep ; 51(1): 738, 2024 Jun 14.
Article in En | MEDLINE | ID: mdl-38874633
ABSTRACT

BACKGROUND:

Interspecific hybrids of rohu (Labeo rohita) and catla (Labeo catla) are common, especially in India due to constrained breeding. These hybrids must segregate from their wild parents as part of conservational strategies. This study intended to screen the hybrids from wild rohu and catla parents using both morphometric and molecular approaches. METHODS &

RESULTS:

The carp samples were collected from Jharkhand and West Bengal, India. The correlation and regression analysis of morphometric features are considered superficial but could be protracted statistically by clustering analysis and further consolidated by nucleotide variations of one mitochondrial and one nuclear gene to differentiate hybrids from their parents. Out of 21 morphometric features, 6 were used for clustering analysis that exhibited discrete separation among rohu, catla, and their hybrids when the data points were plotted in a low-dimensional 2-D plane using the first 2 principal components. Out of 40 selected single nucleotide polymorphism (SNP) positions of the COX1 gene, hybrid showed 100% similarity with catla. Concerning SNP similarity of the 18S rRNA nuclear gene, the hybrid showed 100% similarity with rohu but not with catla; exhibiting its probable parental inheritance.

CONCLUSIONS:

Along with morphometric analysis, the SNP comparison study together points towards strong evidence of interspecific hybridization between rohu and catla, as these hybrids share both morphological and molecular differences with either parent. However, this study will help screen the hybrids from their wild parents, as a strategy for conservational management.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carps / Polymorphism, Single Nucleotide / Hybridization, Genetic Limits: Animals Country/Region as subject: Asia Language: En Journal: Mol Biol Rep Year: 2024 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carps / Polymorphism, Single Nucleotide / Hybridization, Genetic Limits: Animals Country/Region as subject: Asia Language: En Journal: Mol Biol Rep Year: 2024 Document type: Article Affiliation country: