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Catechin-Induced changes in PODXL, DNMTs, and miRNA expression in Nalm6 cells: an integrated in silico and in vitro approach.
Afgar, Ali; Keyhani, Alireza; Afgar, Amirreza; Mirzaei-Parsa, Mohamad Javad; Kermani, Mahdiyeh Ramezani Zadeh; Rezaei, Masoud; Ebrahimipour, Mohammad; Langroudi, Ladan; Bardsiri, Mahla Sattarzadeh; Vahidi, Reza.
Affiliation
  • Afgar A; Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.
  • Keyhani A; Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
  • Afgar A; Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.
  • Mirzaei-Parsa MJ; Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Science, Kerman, Iran.
  • Kermani MRZ; Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.
  • Rezaei M; Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.
  • Ebrahimipour M; Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.
  • Langroudi L; Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
  • Bardsiri MS; Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Science, Kerman, Iran. sabasatarzadeh3261@gmail.com.
  • Vahidi R; Stem Cells and Regenerative Medicine Innovation Center, Kerman University of Medical Sciences, Kerman, Iran. sabasatarzadeh3261@gmail.com.
BMC Complement Med Ther ; 24(1): 234, 2024 Jun 15.
Article in En | MEDLINE | ID: mdl-38879474
ABSTRACT

BACKGROUND:

This study explored the impact of predicted miRNAs on DNA methyltransferases (DNMTs) and the PODXL gene in Nalm6 cells, revealing the significance of these miRNAs in acute lymphocytic leukemia (ALL).

METHODS:

A comprehensive approach was adopted, integrating bioinformatic analyses encompassing protein structure prediction, molecular docking, dynamics, and ADMET profiling, in conjunction with evaluations of gene and miRNA expression patterns. This methodology was employed to elucidate the therapeutic potential of catechin compounds in modulating the activity of DNA methyltransferases (DNMTs) and the PODXL gene.

RESULTS:

The findings from our investigation indicate that catechins possess the capability to inhibit DNMT enzymes. This inhibitory effect is associated with the upregulation of microRNAs miR-200c and miR-548 and a concurrent downregulation of PODXL gene expression. These molecular interactions culminate in an augmented apoptotic response within ALL (Nalm6) cells.

CONCLUSION:

The study posits that catechins may represent a viable therapeutic avenue for inducing apoptosis in ALL cells. This is achieved through the modulation of epigenetic mechanisms and alterations in gene expression profiles, highlighting the potential of catechins as agents for cancer therapy.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Catechin / MicroRNAs Limits: Humans Language: En Journal: BMC Complement Med Ther Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Catechin / MicroRNAs Limits: Humans Language: En Journal: BMC Complement Med Ther Year: 2024 Document type: Article Affiliation country: Country of publication: