PqqF inhibits T6SS secretion by decreasing the pH in Serratia marcescens FS14.
FEMS Microbiol Lett
; 3712024 Jan 09.
Article
in En
| MEDLINE
| ID: mdl-38908910
ABSTRACT
Pyrroloquinoline quinone (PQQ) is a redox cofactor with numerous important physiological functions, and the type VI secretion system (T6SS) is commonly found in Gram-negative bacteria and plays important roles in physiological metabolism of the bacteria. In this study, we found that the deletion of pqqF enhanced the secretion of Hcp-1 in Serratia marcesens FS14 in M9 medium. Transcriptional analysis showed that the deletion of pqqF almost had no effect on the expression of T6SS-1. Further study revealed that the increased secretion of Hcp-1 was altered by the pH changes of the culture medium through the reaction catalyzed by the glucose dehydrogenases in FS14. Finally, we demonstrated that decreased pH of culture medium has similar inhibition effects as PQQ induced on the secretion of T6SS-1. This regulation mode on T6SS by pH in FS14 is different from previously reported in other bacteria. Therefore, our results suggest a novel pH regulation mode of T6SS in S. marcesens FS14, and would broaden our knowledge on the regulation of T6SS secretion.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Serratia marcescens
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Bacterial Proteins
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Culture Media
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PQQ Cofactor
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Type VI Secretion Systems
Language:
En
Journal:
FEMS Microbiol Lett
Year:
2024
Document type:
Article
Affiliation country: