Comparative pharmacokinetics of five primary constituents in Huai-hua powder: a study on normal rats and rats with ulcerative colitis.
J Pharm Pharmacol
; 76(9): 1160-1168, 2024 Sep 03.
Article
in En
| MEDLINE
| ID: mdl-38913100
ABSTRACT
OBJECTIVES:
The goal of this research was to develop a fast, reliable, and sensitive method to simultaneously quantify five key components of Huai-hua Powder (HHP) in rat plasma with genistein served as the internal standard. Furthermore, the established method was used to perform a comparative evaluation of the pharmacokinetic properties of HHP in normal rats and rats with ulcerative colitis (UC).METHODS:
Chromatographic separation was conducted using an ACQUITY HSS T3 column held at a constant temperature of 35°C, with acetonitrile and a 0.1% formic acid solution in water employed as the mobile phases. Multiple-reaction monitoring facilitated MS operation in positive-negative-ion-switching mode. The method's validation demonstrated exceptional linearity (with a correlation coefficient of r ≥ 0.9970), and the validation tests, encompassing precision within and between days, accuracy, recovery, matrix effect, and stability; all met the predefined acceptable criteria. KEYFINDINGS:
The results revealed significant variations in the pharmacokinetic characteristics of the five components between normal and UC rats, suggesting altered drug metabolism rates and extents in the latter group.CONCLUSIONS:
These findings offer crucial scientific insights into the potential clinical application of HHP, particularly in the context of treating UC.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Powders
/
Drugs, Chinese Herbal
/
Colitis, Ulcerative
/
Rats, Sprague-Dawley
Limits:
Animals
Language:
En
Journal:
J Pharm Pharmacol
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: