Depletion of lipid storage droplet-1 delays endoreplication progression and induces cell death in Drosophila salivary gland.
Arch Insect Biochem Physiol
; 116(3): e22132, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38993002
ABSTRACT
Perilipins are evolutionarily conserved from insects to mammals. Drosophila lipid storage droplet-1 (LSD-1) is a lipid storage droplet membrane surface-binding protein family member and a counterpart to mammalian perilipin 1 and is known to play a role in lipolysis. However, the function of LSD-1 during specific tissue development remains under investigation. This study demonstrated the role of LSD-1 in salivary gland development. Knockdown of Lsd-1 in the salivary gland was established using the GAL4/UAS system. The third-instar larvae of knockdown flies had small salivary glands containing cells with smaller nuclei. The null mutant Drosophila also showed the same phenotype. The depletion of LSD-1 expression induced a delay of endoreplication due to decreasing CycE expression and increasing DNA damage. Lsd-1 genetically interacted with Myc in the third-instar larvae. These results demonstrate that LSD-1 is involved in cell cycle and cell death programs in the salivary gland, providing novel insight into the effects of LSD-1 in regulating salivary gland development and the interaction between LSD-1 and Myc.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Salivary Glands
/
Cell Death
/
Drosophila Proteins
/
Larva
Limits:
Animals
Language:
En
Journal:
Arch Insect Biochem Physiol
Journal subject:
BIOLOGIA
/
BIOQUIMICA
Year:
2024
Document type:
Article
Affiliation country: