Vitamin A influences the incretin hormone profiles by activating the retinoic acid receptor ß.
J Diabetes Complications
; 38(8): 108806, 2024 Aug.
Article
in En
| MEDLINE
| ID: mdl-38996583
ABSTRACT
BACKGROUND:
This study aimed to investigate the impact of Vitamin A (VA) on intestinal glucose metabolic phenotypes.METHODS:
Male C57BL/6 mice were randomized assigned to a VA-normal diet (VAN) or a VA-deficient diet (VAD) for 12 weeks. After12 weeks, the VAD mice were given 30 IU/g/d retinol for 10 days and VAN diet (VADN) for 10 weeks. By using glucose tolerance tests, immunofluorescence staining, quantitative polymerase chain reaction, siRNA transduction, and enzyme-linked immunosorbent assay, the glucose metabolic phenotypes as well as secretory function and intracellular hormone changes of STC-1 were assessed.RESULTS:
VAD mice showed a decrease of glucose-stimulated insulin secretion and a loss of intestinal glucagon-like peptide-1 (GLP-1) expression. Through reintroducing dietary VA to VAD mice, the intestinal VA levels, GLP-1 expression and normal glucose can be restored. The incubation with retinol increased VA signaling factors expression within STC-1 cells, especially retinoic acid receptor ß (RARß). The activation of RARß restored intracellular incretin hormone synthesis and secretory function.CONCLUSIONS:
VA deficiency leads to an imbalance of intestinal glucose metabolic phenotypes through a mechanism involving RARß signaling pathway, suggesting a new method to achieve the treatment for VAD induced glucose metabolism impairment.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vitamin A
/
Receptors, Retinoic Acid
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Glucagon-Like Peptide 1
/
Incretins
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Mice, Inbred C57BL
Limits:
Animals
Language:
En
Journal:
J Diabetes Complications
Journal subject:
ENDOCRINOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: