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High-Throughput Assembly of Compositionally Controlled 3D Cell Communities for Developmental Engineering.
Viola, John M; Porter, Catherine M; Gupta, Ananya; Alibekova-Long, Mariia; Prahl, Louis S; Hughes, Alex J.
Affiliation
  • Viola JM; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
  • Porter CM; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
  • Gupta A; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
  • Alibekova-Long M; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
  • Prahl LS; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
  • Hughes AJ; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA. ajhughes@seas.upenn.edu.
Methods Mol Biol ; 2805: 31-50, 2024.
Article in En | MEDLINE | ID: mdl-39008173
ABSTRACT
Cell patterning for 3D culture has increased our understanding of how cells interact among themselves and with their environment during tissue morphogenesis. Building cell communities from the bottom up with size and compositional control is invaluable for studies of morphological transitions. Here, we detail Photolithographic DNA-programmed Assembly of Cells (pDPAC). pDPAC uses a photoactive polyacrylamide gel substrate to capture single-stranded DNA on a 2D surface in large-scale, highly resolved patterns using the photomask technology. Cells are then functionalized with a complementary DNA strand, enabling cells to be temporarily adhered to distinct locations only where their complementary strand is patterned. These temporary 2D patterns can be transferred to extracellular matrix hydrogels for 3D culture of cells in biomimetic microenvironments. Use of a polyacrylamide substrate has advantages, including a simpler photolithography workflow, lower non-specific cell adhesion, and lower stiction to ECM hydrogels during release of patterned hydrogels. The protocol is equally applicable to large (cm)-scale patterns and repetitive arrays of smaller-scale cell interaction or migration experiments.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Hydrogels / Tissue Engineering Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Hydrogels / Tissue Engineering Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Country of publication: