Sample Preparation for X-Ray Fluorescence Microscopy of Iron Distribution in Biological Specimen.
Methods Mol Biol
; 2839: 43-52, 2024.
Article
in En
| MEDLINE
| ID: mdl-39008247
ABSTRACT
Characterizing the two- and three-dimensional distribution of trace metals in biological specimens is key to better understand their role in biological processes. Iron (Fe) is of particular interest in these trace metals due to its widespread role in maintaining cellular health and preventing disease. X-ray fluorescence microscopy (XFM) is emerging as the method of choice for investigators to interrogate the cellular and subcellular distribution of Fe. XFM utilizes the intrinsic X-ray fluorescence properties of each element to produce quantitative 2D and 3D distributions of trace metals within a sample. Herein, methods for sample preparation of cells and tissue for the determination of Fe distribution by XFM are described.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Iron
/
Microscopy, Fluorescence
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA