Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells.
Anal Cell Pathol (Amst)
; 2024: 2165242, 2024.
Article
in En
| MEDLINE
| ID: mdl-39045215
ABSTRACT
This study aimed to construct an eukaryotic expression vector, pEGFP-N1-MIC-1, for overexpressing the mouse macrophage inhibitory cytokine-1 (MIC-1) gene. Additionally, we transfected the MFC cell line to observe the upregulation of MIC-1 gene expression and assess its impact on macrophage phenotype conversion. Enzyme digestion and DNA sequencing confirmed the successful construction of the pEGFP-N1-MIC-1 vector. The transfected MFC cells exhibited a significant increase in MIC-1 protein expression levels. Furthermore, transfection with pEGFP-N1-MIC-1 increased the migration and colony formation capabilities of MFC cells. These results may contribute to future research and the development of therapeutic interventions targeting MIC-1 in macrophages, particularly in the context of gastric cancer.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stomach Neoplasms
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Growth Differentiation Factor 15
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Genetic Vectors
Limits:
Animals
/
Humans
Language:
En
Journal:
Anal Cell Pathol (Amst)
Journal subject:
NEOPLASIAS
/
PATOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: