Protocol for the characterization of the pancreatic tumor microenvironment using organoid-derived mouse models and single-nuclei RNA sequencing.
STAR Protoc
; 5(3): 103203, 2024 Sep 20.
Article
in En
| MEDLINE
| ID: mdl-39058588
ABSTRACT
Single-nuclei RNA sequencing (snRNA-seq) allows for obtaining gene expression profiles from frozen or hard-to-dissociate tissues at the single-nuclei level. Here, we describe a protocol to obtain snRNA-seq data of pancreatic tumors from orthotopically grafted organoid-derived mouse models. We provide details on the establishment of these mouse models, the isolation of single nuclei from pancreatic tumors, and the analysis of the snRNA-seq datasets. For complete details on the use and execution of this protocol, please refer to Mucciolo et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pancreatic Neoplasms
/
Organoids
/
Sequence Analysis, RNA
/
Tumor Microenvironment
Limits:
Animals
/
Humans
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Country of publication: